—Confocal microscopy is a method for induced fluorescence detecting system which is widely used in biochip and microfluidic chip. However, the sensitivity is influenced seriously by the installation uncertainty which leads to the detecting plane being away from the object plane. A new autofocus confocal system with an electrically tunable liquid lens is designed to adjust the detecting plane automatically through changing the focal length of the object electrically tunable lens group until light focus on the center of detection channel by a camera. This design makes it possible to improve the accuracy of confocal microscopy in a small space.
—Confocal microscopy, electrically tunable liquid lens, automatic focusing.
The authors are with the School of Optoelectronics, Beijing Institute of Technology, Beijing, 100081, China (e-mail: email@example.com, firstname.lastname@example.org, email@example.com).
Cite: Chang Zhou, Xiaoming Hu, and Ya Zhou, "An Induced Fluorescence Detecting System with Autofocus Electrically Tunable Len," International Journal of Engineering and Technology vol. 8, no. 4, pp. 297-300, 2016.