Abstract—Integrated membrane process (IMP) and alginate affinity precipitation (AAP) were assessed for the purification of Aspergillus carbonarius polygalacturonase (PG) obtained after submerged (SmF) and solid-state fermentations (SSF). IMP enhanced the purity (4.69 fold) and recovery (76%) of SmF-PG, but did not improve the purity of SSF and commercial PG due to the presence of other similar molecular mass proteins. AAP enhanced the PG purity and recovery in all the PG samples (SmF, SSF and commercial PG). IMP offers several process advantages over AAP but not suitable for purification of all types of PGs. Our studies on SmF- and SSF-PG had shown that downstream process needs are specific for individual cases and warrants careful considerations.
Index Terms—Alginate affinity precipitation, integrated membrane process, polygalacturonase, submerged and solid-state fermentations.
Cite : E. Nakkeeran, R. Subramanian and S. Umesh Kumar , " Process Assessment for the Purification of Aspergillus carbonarius Polygalacturonase Produced by Submerged and Solid-State Fermentations ," International Journal of Engineering and Technology vol. 1, no. 4, pp. 278-281, 2009.