Abstract—Ribonucleases (RNase) are important analytical enzymes and widely distributed in nature. RNase from microorganisms widely used in molecular biology study, food, and pharmaceutical industry. As an important analytical tool, they have played a major role in study on the structure and function of RNA. In single cell protein production, they are used to remove RNA in cell. RNases have also been applied commercially to produce nucleotides for clinical use or for seasoning nucleotides. Many rib onucleases are highly cytotoxic. They have several important biological roles such as antitumor and antiviral activity. The selected strain Aspergillus niger ATCC 26550 is chosen for extra cellular RNase enzyme production by maintaining the culture medium at 30°C, pH 5.5 where the enzyme production was found to be growth associated. Beef extract, peptone and ammonium sulfate are the superior nitrogen source. Addition of Mg2+, Ca2+ to the growth medium significantly enhanced the enzyme production. Glucose found to be the excellent carbon source for RNase production. This method of process optimization is the classical approach of one factor at a time.
Index Terms—Aspergillus niger, enzyme activity, medium optimization, ribonuclease.
Cite: Gundampati R.K and Debnath M, " Extracellular Ribonuclease from Aspergillus niger: process optimization for production," International Journal of Engineering and Technology vol. 1, no. 4, pp. 317-320, 2009.