Abstract—Cryopreservation of germplasm at liquid nitrogen (-196°C) is a perfect method for the long-term conservation of plant genetic resources. A cryopreservation process using dehydration was performed for seeds of lily [Lilium Iedebourii (Baker) Bioss.]. Seeds were subjected to a rapid freezing protocol in liquid nitrogen following dehydration and treatment with 0.75 M sucrose for 1 h. Survival after freezing was nil for control seeds and, 75% for seeds treated with sucrose and dehydration.
Index Terms—conservation, dehydration, germplasm, lily, sucrose.
B. Kaviani Livani is with the Department of Horticultural Science, Faculty of Agriculture, Islamic Azad Universiy, Rasht Branch, Rasht, Iran
Phone: +989111777482, +981314240149; Fax: +981313462255
Cite: B. Kaviani Livani , "Cryopreservation of Seeds of Lily [Lilium Iedebourii (Baker) Bioss.]: Use of Sucrose and Dehydration ," International Journal of Engineering and Technology vol. 1, no. 4, pp. 291-292, 2009.