Abstract—In the present research work, a immobilized tannase system was used to produce gallic acid from two sources, methyl gallate and tannic acid. Tannase was obtained by solid-state culture (SSC) using the strain of A. niger GH1. The tannase extract was concentrated with polyethylene glycol 6000 at 4°C for 12 h. Tannase activity was assayed by a HPLC method. The tannase activity detected in the concentrated extract was almost 40 times higher than the activity in the extract crude. The results obtained in the degradation of tannic acid by the immobilized tannase showed that the concentration of gallic acid was higher (0,387 g/L) than that obtained with methyl gallate (0,199 g /L). Production of gallic acid with an enzymatic process is an attractive alternative that permits the reuse of the biocatalyst.
Index Terms—Tannase, Gallic Acid, Immobilization
K. Cruz-Aldaco, E. Rivas-Martinez, R. Rodríguez-Herrera and C.N. Aguilar are with the Food Research Department. School of Chemistry. Universidad Autonoma de Coahuila, Saltillo, 25280, Coahuila, Mexico. (Corresponding author, C.N. Aguilar: 53 844 4161238, fax: 52 844 4159534;).
Cite : Karina Cruz-Aldaco, Erika Rivas-Martínez, Raúl Rodríguez-Herrera & Cristóbal Noe Aguilar, " Immobilization of Aspergillus niger GH1 Tannase for the Production of the Antioxidant Gallic Acid," International Journal of Engineering and Technology vol. 1, no. 4, pp. 288-290, 2009.