Abstract—The goal of this study was the development and optimization of a multiplex-PCR protocol for the simultaneous detection of three accompanying transgenic sequences. The DNA was extracted and the target sequences were separately detected by PCR. Multiplex PCR was performed with DNA from maize, cotton and soybean for the detection of transgenic sequences, allowing the amplification of nos (125 bp), ntpII (271 bp) and luc (450 bp) at 62º C. Optimizing the multiplex PCR technique for the amplification of the accompanying transgenic sequences could become a very valuable tool for detecting genetically modified organisms (GMO).
Index Terms—nos, luc, ntpII
Claudia Gabriela Macías de la Cerda, Raúl Rodríguez Herrera, Cristóbal Noé Aguilar González is with the Food Research Department, School of Chemistry, Universidad Autónoma de Coahuila Saltillo, MX-25 280 Coahuila, Mexico.
Manuel Humberto Reyes Valdés is with the Department of Plant Breeding Universidad Autónoma Agraria 'Antonio Narro' Buenavista, Saltillo, MX-25 000 Coahuila, México
Cite: Claudia Gabriela Macías de la Cerda, Raúl Rodríguez Herrera, Cristóbal Noé Aguilar González and Manuel Humberto Reyes Valdés, "Multiplex-PCR Detection of Accompanying GMO Transgenic Sequences," International Journal of Engineering and Technology vol. 1, no. 4, pp. 285-287, 2009.